UPLC™: An Introduction and Review
Top Cited Papers
- 1 April 2005
- journal article
- Published by Taylor & Francis in Journal of Liquid Chromatography & Related Technologies
- Vol. 28 (7-8) , 1253-1263
- https://doi.org/10.1081/jlc-200053046
Abstract
Ultra performance liquid chromatography™ (UPLC) takes advantage of technological strides made in particle chemistry performance, system optimization, detector design, and data processing and control. Using sub‐2 µm particles and mobile phases at high linear velocities, and instrumentation that operates at higher pressures than those used in HPLC, dramatic increases in resolution, sensitivity, and speed of analysis can be obtained. This new category of analytical separation science retains the practicality and principles of HPLC while creating a step‐function improvement in chromatographic performance. This review introduces the theory of UPLC, and summarizes some of the most recent work in the field.Keywords
This publication has 9 references indexed in Scilit:
- Very high pressure HPLC with 1 mm id columnsThe Analyst, 2004
- Fast LC/MS in the analysis of small moleculesAnalytical and Bioanalytical Chemistry, 2003
- Very High Pressure Gradient LC/MS/MSAnalytical Chemistry, 2001
- Practical aspects of ultrahigh pressure capillary liquid chromatographyJournal of Chromatography A, 2001
- Synthesis of spherical porous silicas in the micron and submicron size range: challenges and opportunities for miniaturized high-resolution chromatographic and electrokinetic separationsPublished by Elsevier ,2000
- Fast ultrahigh-pressure liquid chromatography: On-column UV and time-of-flight mass spectrometric detectionJournal of Microcolumn Separations, 1999
- Ultrahigh-Pressure Reversed-Phase Liquid Chromatography in Packed Capillary ColumnsAnalytical Chemistry, 1997
- Ultimate limits in high-pressure liquid chromatographyJournal of Chromatography A, 1975
- Longitudinal diffusion and resistance to mass transfer as causes of nonideality in chromatographyChemical Engineering Science, 1956