IN VITRO SYNTHESIS OF PHYTOCHROME APOPROTEIN DIRECTED BY mRNA FROM LIGHT AND DARK GROWN AVENA SEEDLINGS*

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Abstract
Abstract—The aim of this work was to compare the translatability of poly‐A‐RNA from light‐ and dark‐grownArenaseedlings, the product of which should be phytochrome.Polysomal poly‐A‐RNA was isolated from 4‐day‐old dark‐grownAvenaseedlings(Avena sativaL. cv. Garry) and translated in a rabbit reticulocyte lysate system. Immunoprecipitation by anti‐phytochrome serum was used for measurements of specific phytochrome translation. Characterization of the translated and immunoprectpitated protein was performed by comparison with [35S]‐methioninein vivo‐labelled phytochrome. The specificity of the precipitation was shown by parallel use of non‐specific serum and by competitive inhibition of precipitation by exogenous unlabelled phytochrome isolated by affinity chromatography.