Demonstration of Interference between Dengue Virus Types in Cultured Mosquito Cells using Monoclonal Antibody Probes

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Abstract
Cultured A. albopictus cells (clone C6/36), persistently infected (PI) with dengue virus type 1 (dengue-1) were found resistant to superinfection with dengue virus type 3 (dengue-3). This was determined by indirect immunofluorescent (IF) staining of cultures using monoclonal antibody against a dengue-3 type-specific antigen. Dengue-1 PI cultures stained with this antibody 3 days after superinfection with dengue-3 virus (multiplicity of infection of 2) had dengue-3 antigen in 0.1-1.0% of the cells. Control cultures infected with dengue-3 at the same multiplicity contained dengue-3 antigen in > 90% of the cells. The resistance to superinfection was not interferon-mediated and occurred within 20 h after primary infection. In cultures simultaneously infected with 2 dengue virus types, 1 virus type was excluded from replication in most cells. A small population of cells was also found (.apprx. 1%) that contained type-specific antigen of both dengue virus types.