Three-dimensional imaging with optical tweezers
- 1 November 1999
- journal article
- Published by Optica Publishing Group in Applied Optics
- Vol. 38 (31) , 6597-6603
- https://doi.org/10.1364/ao.38.006597
Abstract
We demonstrate a three-dimensional scanning probe microscope in which the extremely soft spring of an optical tweezers trap is used. Feedback control of the instrument based on backscattered light levels allows three-dimensional imaging of microscopic samples in an aqueous environment. Preliminary results with a 2-µm-diameter spherical probe indicate that features of approximately 200 nm can be resolved, with a sensitivity of 5 nm in the height measurement. The theoretical resolution is limited by the probe dimensions.Keywords
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