Role of hydrogen peroxide in the cytotoxicity of the xanthine/xanthine oxidase system
- 15 January 1988
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 249 (2) , 391-399
- https://doi.org/10.1042/bj2490391
Abstract
1. The survival of mammalian epithelial cells exposed in vitro to the xanthine/xanthine oxidase system in phosphate-buffered saline (PBS) or serum-containing medium (SCMEM) was investigated. 2. The cytotoxic effect observed depended on the composition of the medium in which the enzymic reaction was carried out; a surviving fraction of 5 x 10(-5) was found for cells exposed in PBS and 5.2 x 10(-1) for those in SCMEM. 3. The cytotoxic product(s) formed by the xanthine/xanthine oxidase system was relatively stable in PBS; survival of cells incubated after completion of the enzymic reaction was always less than that found for cells exposed during the reaction in the same system. 4. Superoxide dismutase or mannitol present during the enzymic reaction did not inhibit the cytotoxic effect. 5. NaN3 (a single-oxygen quencher and a catalase inhibitor) added to the system in SCMEM caused a reduction in survival to the level observed for cells exposed to the enzymic reaction in PBS. 6. Catalase completely protected cells, but no protection was observed when both catalase and NaN3 were present in the reaction mixture. 7. A similar cytotoxic effect was produced when cells were treated with H2O2 alone. 8. The rate of H2O2 decomposition in medium was accelerated by the presence of serum, but this was completely inhibited by NaN3. 9. It is concluded that H2O2 is the major cytotoxic product formed by the xanthine/xanthine oxidase system.This publication has 48 references indexed in Scilit:
- Oxidation of cell wall polysaccharides by hydrogen peroxide: A potential mechanism for cell wall breakdown in plantsBiochemical and Biophysical Research Communications, 1986
- Oxidants increase paracellular permeability in a cultured epithelial cell line.Journal of Clinical Investigation, 1985
- Superoxide dismutase inhibits the superoxide‐driven Fenton reaction at two different levelsFEBS Letters, 1985
- Hydroxyl free radical reactions with amino acids and proteins studied by electron spin resonance spectroscopy and spin-trappingBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1984
- Formation of long-lived hydroxyl free radical adducts of proline and hydroxyproline in a fenton reactionBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1984
- The Role of Active Oxygen (102 and 0·2-) Induced by Crude Coal Tar and Its Ingredients Used in Photochemotheraphy of Skin DiseasesJournal of Investigative Dermatology, 1984
- A possible mechanism of the generation of singlet molecular oxygen in NADPH-dependent microsomal lipid peroxidationBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1976
- The mechanism of liver microsomal lipid peroxidationBiochimica et Biophysica Acta (BBA) - General Subjects, 1975
- The role of superoxide and singlet oxygen in lipid peroxidation promoted by xanthine oxidaseBiochemical and Biophysical Research Communications, 1973
- The microestimation of succinate and the extinction coefficient of cytochrome cBiochimica et Biophysica Acta, 1959