Splicing enhances translation in mammalian cells: an additional function of the exon junction complex
Top Cited Papers
Open Access
- 15 January 2004
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 18 (2) , 210-222
- https://doi.org/10.1101/gad.1163204
Abstract
In mammalian cells, spliced mRNAs yield greater quantities of protein per mRNA molecule than do otherwise identical mRNAs not made by splicing. This increased translational yield correlates with enhanced cytoplasmic polysome association of spliced mRNAs, and is attributable to deposition of exon junction complexes (EJCs). Translational stimulation can be replicated by tethering the EJC proteins Y14, Magoh, and RNPS1 or the nonsense-mediated decay (NMD) factors Upf1, Upf2, and Upf3b to an intronless reporter mRNA. Thus, in addition to its previously characterized role in NMD, the EJC also promotes mRNA polysome association. Furthermore, the ability to stimulate translation when bound inside an open reading frame appears to be a general feature of factors required for NMD.Keywords
This publication has 73 references indexed in Scilit:
- Y14 and hUpf3b Form an NMD-Activating ComplexMolecular Cell, 2003
- Positive and Negative Effects of the Major Mammalian Messenger Ribonucleoprotein p50 on Binding of 40 S Ribosomal Subunits to the Initiation Codon of β-Globin mRNAPublished by Elsevier ,2002
- An extensive network of coupling among gene expression machinesNature, 2002
- Messenger-RNA-binding proteins and the messages they carryNature Reviews Molecular Cell Biology, 2002
- The DExH/D box protein HEL/UAP56 is essential for mRNA nuclear export in DrosophilaCurrent Biology, 2001
- Drosophila Y14 shuttles to the posterior of the oocyte and is required for oskar mRNA transportCurrent Biology, 2001
- Eukaryote-specific Domains in Translation Initiation Factors: Implications for Translation Regulation and Evolution of the Translation SystemGenome Research, 2000
- The compartmentalization of protein synthesis: Importance of cytoskeleton and role in mRNA targetingThe International Journal of Biochemistry & Cell Biology, 1996
- Identification and characterisation of a novel human RNA-binding proteinGene, 1995
- Effect of viral infection on host protein synthesis and mRNA association with the cytoplasmic cytoskeletal structure.The Journal of cell biology, 1985