The gene for human lymphocyte homing receptor is located on chromosome 11

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Abstract
The mouse monoclonal antibody Hermes‐3 recognizes the human lymphocyte homing receptor. A panel of mouse‐human T lymphocyte hybrids, carrying all mouse chromosomes and a limited number of human chromosomes, was analyzed for expression of human homing receptor by indirect immunofluorescence and immuno‐precipitation of radiolabeled cell lysates with Hermes‐3 antibody. Karyotypic analysis of the tested clones showed that the expression of human homing receptor correlated to the presence of human chromosome 11 in all but one clone. However, con‐canavalin A induced a weak to moderate expression of the homing receptor in this clone, but not in a chromosome 11‐ clone. Another clone, heterogeneous for the expression of homing receptor, was separated into a Hermes‐3 and a Hermes‐3‐ fraction with a fluorescence‐activated cell sorter. Karyotypic analysis performed after sorting showed human chromosome 11 to segregate with the Hermes‐3 antigen. To confirm these data we correlated the expression of two chromosome 11‐coded antigens, Trop‐4 and Leu‐7, with the expression of the homing receptor. In our hybrid clones these three antigens were expressed concordantly. The gene coding for the human lymphocyte homing receptor recognized by Hermes‐3 is thus assigned to chromosome 11.