Effects of diacylglycerol and phorbol ester on acetylcholine release and action at the neuromuscular junction in mice

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Abstract
Miniature endplate potentials and nerve‐evoked endplate potentials were studied in a phrenic nerve‐diaphragm preparation of the mouse. A phorbol ester, phorbol‐12β,13α‐dibutyrate, and a diacylglycerol, 1‐oleyl‐2‐acetyl‐sn‐glycerol, both increased the frequency of miniature endplate potentials. The effect of the phorbol ester on the frequency was still significant when the preparation was incubated in a calcium‐deficient solution. The phorbol ester, but not the diacylglycerol, caused a significant increase in the amplitude of the miniature potentials. The phorbol ester also increased the amplitude of the endplate potentials in the presence of (+)‐tubocurarine. The mechanisms of action of phorbol ester and diacylglycerol are discussed in relation to the control of acetylcholine release and action at the neuromuscular junction.

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