RECIPROCAL CORRELATION BETWEEN EXPRESSION OF P-GLYCOPROTEIN AND ACCUMULATION OF RHODAMINE-123 IN HUMAN TUMORS

  • 1 November 1989
    • journal article
    • research article
    • Vol. 9  (6) , 1633-1637
Abstract
The aim of this investigation was to determine whether a correlation exists between expression of the multidrug-resistance associated P-glycoprotein 170 (P-gp170) and the accumulation of rhodamine 123 in human tumors. We investigated a panel of inherently resistant cell lines originating from human kidney tumor specimens and chemotherapeutically pretreated leukemias. The expression of P-gp170 was evaluated by means of two different immunohistological methods using two monoclonal antibodies against P-gp170. We found a significant reciprocal correlation between expression of P-gp170 and accumulation of rhodamine 123. This supports the view that P-gp170 might function as efflux pump in human tumors, as previously described for experimental models.