Standardization of lymphocyte transformation to phytohaemagglutinin.
- 1 July 1973
- journal article
- Vol. 14 (3) , 457-67
Abstract
In contrast to the million cells used by many current workers, careful analysis of lymphocyte transformation to phytohaemagglutinin determined optimum conditions as (i) 200,000 lymphocytes in 1 ml of culture fluid in 100 × 12-mm round-bottomed glass tubes (ii) phytohaemagglutinin at 1 μg/tube (iii) 3H-thymidine at 1 μCi/tube (iv) with a convenient incorporation time 4 hr (v) starting 44 hr after adding phytohaemagglutinin. It was then found 10% autologous serum was the optimum concentration in the culture fluid. The log–normal range of transformation indices for young adults was 27–93–255.This publication has 12 references indexed in Scilit:
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