Partition of macromolecules and cell particles in aqueous two-phase systems based on hydroxypropyl starch and poly(ethylene glycol)
- 1 December 1990
- journal article
- research article
- Published by Springer Nature in Applied Biochemistry and Biotechnology
- Vol. 26 (3) , 281-295
- https://doi.org/10.1007/bf02921507
Abstract
The partition behavior of proteins, nucleic acids, cell membranes, cell organelles and whole cells has been studied in liquid-liquid two-phase systems composed of water, poly-(ethylene glycol), and an hydroxypropyl starch. The properties of the systems are in many respects comparable with the traditional poly(ethylene glycol)-dextran systems, but the cost is reduced to around one-fifth.This publication has 26 references indexed in Scilit:
- Affinity liquid‐liquid extraction of lactate dehydrogenase on a large scaleBiotechnology & Bioengineering, 1987
- Fractionation of proteins from human serum by counter-current distributionJournal of Biotechnology, 1987
- Aqueous polymer two-phase systems in biotechnologyFluid Phase Equilibria, 1986
- New aqueous two-phase system based on hydroxypropyl starch useful in enzyme purificationEnzyme and Microbial Technology, 1986
- Preparation of cibacron blue F3G‐A (polyethylene glycol) in large scale for use in affinity partitioningBiotechnology & Bioengineering, 1985
- A Process for large‐scale isolation of β‐galactosidase from E. coli in an aqueous two‐phase systemBiotechnology & Bioengineering, 1983
- Evaluation of crude dextran as phase-forming polymer for the extraction of enzymes in aqueous two-phase systems in large scaleBiotechnology & Bioengineering, 1982
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Separation of subchloroplast membrane particles by counter-current distributionBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1976