Simultaneous Extraction and Determination of Oxolinic Acid and Flumequine in Fish Silage by HPLC

Abstract
A simple and rapid method for the simultaneous extraction and determination of residues of oxolinic acid (OX) and flumequine (FQ) in fish silage, is presented. The samples were extracted with acetone and ammonia. When applying traditional liquid-liquid extraction, clean extracts were obtained, the recovery being 95.3–96.2 % for OX and 96.9–99.2 % for FQ. The detection limits were 40 ng/g for OX and 50 ng/g for FQ.

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