A second type II restriction endonuclease fromThermus aquaticuswith an unusual sequence specificity

Open Access

25 July 1984

journal article
research article
Published by Oxford University Press (OUP) in Nucleic Acids Research

Vol. 12 (14) , 5567-5581
https://doi.org/10.1093/nar/12.14.5567

Abstract

A Type II restriction endonuclease activity free of TaqI was prepared from Therraus Aquatious YT. The fraction contains two endonucleolytic components with apparently different specificities, however the major activity is sufficiently dominant to allow partial digestion analysis of the position of recognition sites. A precise determination of the location of cleavage sites in pBR322 DNA and a computer-aided search for regions of homology in the vicinity of the cut sites indicate that this enzyme recognizes the nonpalindromic sequences GACCGA or CACCCA. Other related sequences are not cleaved, in particular, GACCCA and CACCGA, indicating that the enzyme requires the identity of nucleotides in the first and fifth positions, a type of specificity that has not been previously reported. The position of cleavage is located outside of the site and is represented as:

Keywords

This publication has 18 references indexed in Scilit:

[57] Sequencing end-labeled DNA with base-specific chemical cleavages
Published by Elsevier ,2004
[48] A general method for defining restriction enzyme cleavage and recognition sites
Published by Elsevier ,1980
Complete Nucleotide Sequence of the Escherichia coli Plasmid pBR322
Published by Cold Spring Harbor Laboratory ,1979
Organization and Transcription of the Simian Virus 40 Genome
Published by Springer Nature ,1979
Rapid, single-step purification of restriction endonucleases on Cibacron Blue F3GA-agarose
Biochemistry, 1978
DNA sequencing with chain-terminating inhibitors
Proceedings of the National Academy of Sciences, 1977
A thermostable sequence-specific endonuclease from Thermus aquaticus.
Proceedings of the National Academy of Sciences, 1977
A simple, general procedure for purifying restriction endonucleases
Nucleic Acids Research, 1977
A simple method for DNA restriction site mapping
Nucleic Acids Research, 1976
The Mapping and sequence determination of the single site in φX174am3 replicative form DNA cleaved by restriction endonuclease Pst I
FEBS Letters, 1976