Unique expression pattern of protein kinase C‐θ: high mRNA levels in normal mouse testes and in T‐lymphocytic cells and neoplasms
- 1 July 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 326 (1-3) , 51-55
- https://doi.org/10.1016/0014-5793(93)81759-s
Abstract
A 2.2-kb cDNA that contains the entire coding region of mouse protein kinase C-θ (PKC-θ) was cloned from skeletal muscle mRNA using reverse transcription and the polymerase chain reaction (PCR). This clone was used as a probe to study the expression of this PKC isoform in normal and transformed hemopoietic cells and other normal tissues. By far the highest steady-state level of PKC-0 MRNA was found as a 2.8-kb transcript on a Northern blot of poly(A)+ RNA from testes. High levels were also found in skeletal muscle, spleen, T lymphomas and purified normal T lymphocytes, but these tissues and cells expressed two transcripts, 3.3 kb and 3.8 kb. Lower levels of similar size transcripts were found in normal brain, B lymphocytes and B-lymphocytic tumors and cell lines.Keywords
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