SUBSTITUTION OF KNOWN COMPOUNDS FOR ASCITIC FLUID IN THE CULTIVATION OFBORRELIA VINCENTII
- 1 June 1959
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 77 (6) , 800-803
- https://doi.org/10.1128/jb.77.6.800-803.1959
Abstract
The ascitic fluid supplement normally required for the growth of Borrelia vincentii in a veal heart infusion medium can be replaced by a mixture of 5 coenzymes (cocarboxylase, codecarboxylase, coenzyme A, adenosine triphosphate, and diphosphopyridine nucleotide) and glucose-1-phosphate. The organism reached maximal growth through 12 successive transfers in this medium. Further, 4 strains of oral treponemes and 3 additional strains of B. vincentii reached maximal growth through 2 transfers in the same medium; however, 3 strains of Borrelia buccalis did not survive under these conditions.Keywords
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