Untersuchungen zur enzymatischen Bräunung bei Kartoffeln (Solanum tuberosum)
- 1 January 1977
- journal article
- abstracts
- Published by Springer Nature in Zeitschrift für Lebensmittel-Untersuchung und Forschung
- Vol. 163 (2) , 92-95
- https://doi.org/10.1007/bf01126024
Abstract
31 Proben von schwach, mittelstark und stark bräunenden Kartoffelsorten wurden untersucht. Bei der Elektrophorese in Polyacrylamid-gel zeigten unterschiedlich bräunende Sorten charakteristische Phenoloxydase-Muster, die sich hauptsächlich in den Intensitäten der Enzym-Banden unterscheiden. Die qualitative Bestimmung der Phenole ergab keine wesentlichen Sortenunterschiede. Tyrosin, Chlorogensäure und Kaffeesäure liefern gefärbte Oxydationsprodukte. Nur in Anwesenheit von Tyrosin wurden die charakteristischen Farbstufen der Bräunung in vivo beobachtet. Daraus ist zu schließen, daß der Verfärbung bei allen Sorten die gleichen Reaktionen zugrunde liegen. 31 samples of potato varieties with slow, medium and fast rates of browning were studied. Characteristic enzyme patterns were obtained from polyacrylamide gel electrophoresic of the phenoloxidases of varieties with different discolouration rates. The differences lie mainly in the intensities of the enzyme bands. The qualitative determination of the phenols showed no significant differences. Tyrosine, chlorogenic acid and caffeic acid produce coloured oxidation products; the characteristic colour gradations of in vivo browning were only observed in the presence of tyrosine. It is concluded that the same reactions take place duriiDg the discolouration of all the varieties.Keywords
This publication has 14 references indexed in Scilit:
- Multiple forms of soluble monophenol, dihydroxyphenylalanine: oxygen-oxidoreduetase (EC 1.14.1$.1) from potato tubers (Solanum tuberosum)Zeitschrift für Lebensmittel-Untersuchung und Forschung, 1975
- Enzymic browning and free tyrosine in potatoes as affected by level of treatment with pentachloronitrobenzeneJournal of Agricultural and Food Chemistry, 1969
- Anatomy and compositional variation within potatoes II. Phenolics, enzymes and other minor componentsAmerican Journal of Potato Research, 1969
- Enzymic browning and free tyrosine in potatoes as affected by pentachloronitrobenzeneJournal of Agricultural and Food Chemistry, 1968
- Eine Objektive Methode zur Bestimmung der Rohverfärbung von Kartoffeln mit Hilfe Eines LeukometersPotato Research, 1966
- Influence of variety, cultural conditions and temperature of storage on enzymic browning of potato tubersJournal of the Science of Food and Agriculture, 1963
- The action of potato phenolase on flavonoid compoundsJournal of the Science of Food and Agriculture, 1959
- Colorimetric determination of tyrosineBiochemical Journal, 1957
- The identification of coumarins and related compounds by filter-paper chromatographyBiochemical Journal, 1953
- 555. The flavonoid glycosides of Dahlia variabilis. Part I. General introduction. Cyanidin, apigenin, and luteolin glycosides from the variety “ Dandy.”Journal of the Chemical Society, 1953