Nature and Number of Ground-Beef Microorganisms Capable of Growth at 25°C but Not at 32°C

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Abstract
The nature and relative population density of microorganisms capable of growth at 25°C but not at 32°C was determined on enumerations from ground beef. Only approximately 60% as many bacteria were recovered when incubation was at the higher temperature. One-third of the randomly selected isolates from the 25°C plates were unable to grow at 32°C. Some of these isolates were strikingly similar to pathogens. Incubation of plates at 25°C for 48 h is recommended to improve recovery of bacteria of significance in ground beef.