Root Hair Cell Enhancement in Tissue Cultures from Soybean Roots: A Useful Model System
- 1 January 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 59 (1) , 97-102
- https://doi.org/10.1104/pp.59.1.97
Abstract
A technique for obtaining large numbers of root hair cells in cell cultures from soybeans [Glycine max] is described. The cells were grown on agar containing the Prairie Regional Laboratory B5 (PRL-B5) medium for periods longer than 60 days. Mixed populations of cultured root hair cells and cortical cells were used to study the in vitro association between soybean cells and Rhizobium japonicum. The advantages of these types of root cell cultures in studies of symbiosis are discussed.This publication has 7 references indexed in Scilit:
- Reduction of acetylene by stationary cultures of free-living Rhizobium sp. under atmospheric oxygen levelsCanadian Journal of Microbiology, 1976
- Synergetic Cultures of Glycine max Root Cells and Rhizobia Separated by Membrane FiltersPlant Physiology, 1976
- Acetylene reduction by transfilter suspension cultures of Rhizobium japonicumBiochemical and Biophysical Research Communications, 1975
- A Simple Technique for the Establishment of Nitrogenase in Soybean Callus CulturePlant Physiology, 1974
- Factors Affecting the Reduction of Acetylene by Rhizobium-Soybean Cell Associations in VitroPlant Physiology, 1974
- Nutrient requirements of suspension cultures of soybean root cellsExperimental Cell Research, 1968