Generation of a transmembrane gradient of Na+ in Methanosarcina barkeri
- 1 January 1987
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 162 (2) , 461-466
- https://doi.org/10.1111/j.1432-1033.1987.tb10624.x
Abstract
A transmembrane Na+ gradient was generated by Methanosarcina barkeri during methanogenesis. The intracellular Na+ concentration amounted to approximately one fifth of the extracellular one. A secondary Na+/H+ antiport system was shown to be responsible for Na+ extrusion. This system could be inhibited by amiloride. In the presence of amiloride the ΔpH across the cytoplasmic membrane increased and a transmembrane Na+ gradient could neither be generated nor maintained. The possible role of Na+ in the oxidation of methanol to the level of formaldehyde is discussed.Keywords
This publication has 22 references indexed in Scilit:
- An electrogenic sodium‐translocating ATPase in Methanococcus voltaeFEBS Letters, 1986
- Sodium bioenergeticsTrends in Biochemical Sciences, 1984
- Kinetic properties of sodium-proton antiport in Escherichia coli membrane vesicles: effects of imposed electrical potential, proton gradient, and internal pHBiochemistry, 1984
- Sodium‐dependent isoleucine transport in the methanogenic archaebacterium Methanococcus voltaeFEBS Letters, 1984
- Na+/H+ antiportersBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1983
- Sodium dependence of methane formation in methanogenic bacteriaFEBS Letters, 1982
- The Generation of an Electrochemical Gradient of Sodium Ions upon Decarboxylation of Oxaloacetate by the Membrane–Bound and Na+–Activated Oxaloacetate Decarboxylase from Klebsiella aerogenesEuropean Journal of Biochemistry, 1982
- pH homesstasis in bacteriaBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1981
- The role of Na+ in transport processes of bacterial membranesBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1979
- Evidence for ATP synthesis driven by a proton gradient in MethanosarcinabarkeriBiochemical and Biophysical Research Communications, 1978