Somatic embryogenesis in Nicotiana tabacum L.: induction by thidiazuron of direct embryo differentiation from cultured leaf discs
- 1 January 1993
- journal article
- Published by Springer Nature in Plant Cell Reports
- Vol. 12 (3) , 154-159
- https://doi.org/10.1007/bf00239097
Abstract
Induction of somatic embryogenesis by different growth regulators was examined in leaf disc cultures of Nicotiana tabacum L. Direct differentiation of somatic embryos occurred on media supplemented with naphthaleneacetic acid (NAA) and N6-benzylaminopurine (BAP). Thidiazuron (N-phenyl-N′-1, 2,3,-thiadiazol-5-ylurea; TDZ) not only substituted for the most effective NAA-BAP combination but also induced a higher frequency of somatic embryogenesis. Regenerated somatic embryos were capable of developing into plants.Keywords
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