Electro-Immortalization of B Lymphocytes Isolated from Stomach Carcinoma Biopsy Material
- 1 April 1993
- journal article
- research article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 12 (2) , 221-225
- https://doi.org/10.1089/hyb.1993.12.221
Abstract
The advantages of electrofusion were used to immortalise the small number of B-cells from fresh biopsy material taken from a gastric carcinoma of a patient. Two stable human antibody secreting clones could be produced which exhibited functional activity against the autologous tumour cells (inhibition of cell adhesion and immunofluorescence staining of the membranes). This shows that a variety of hitherto inacessible B lymphocyte populations from other human organ biopsies can be immortalised by the improved electrofusion technigue.Keywords
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