Studies on phospholipids. 5. The separation of dinitrophenylated and methylated phospholipids by countercurrent distribution

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Abstract
Countercurrent methods have been applied to the separation of dinitrophenylated and methylated phospholipids from rat liver and sheep brain. Three minor phospholipids have been characterized from rat-liver lipids, two of which resemble phosphatidic acids. Three different forms of unknown amino-phospholipid have been distinguished by their partition coefficients (k) in CCl4 methanol-water. Rat-liver lipids contain two with k 2.5 and 8.0 and the ratio dinitrophenyl groups/P equal to 0.5 and 0.67 respectively. Sheep-brain lipids contain phospholipids with k 1.0 and 8.0 and the ratio dinitrophenyl groups/P equal to 0.5 and 0.67 respectively, and the material with k 1.0 also contains choline choline/P 0.48).