Studies on phospholipids. 1. The determination of amino nitrogen in unhydrolysed phospholipids

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Abstract
1-Fluoro-2,4-dinitrobenzene reacts in the presence of triethylamine with the free amino groups of phospholipids dissolved in benzene. This reaction serves as the basis of a method for the quantitative estimation of the free amino groups in the phospholipids. The method is considered to be more reliable than the ninhydrin method in that the reagent is stable, the blank negligible and the need for a standard is eliminated. As little as 0.02 mole can be estimated, and 01-2.5 moles represents the usual range. Lecithin and other lipids do not interfere but with low concentrations of cephalin, special precautions are necessary.