Effect of Fluoride on Glycolytic and Citric Acid Cycle Metabolites in Rat Liver

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Abstract
The effect of the intraperitoneal injection of 2 mg of fluoride or the ingestion of diets containing 450 or 600 ppm fluoride on the concentrations of various rat liver glycolytic and citric acid cycle metabolites was studied. Fluoride injection substantially increased liver fluoride, and caused changes in metabolite levels which were compatible with an inhibition of enolase. The ingestion of dietary fluoride caused a decrease in the level of most glycolytic intermediates, particularly pyruvate and lactate, and a large increase in liver citrate. Fluoride ingestion was also shown to be associated with a decreased activity of liver pyruvic kinase, but it did not influence liver enolase activity. With the exception of the increase in citrate, the changes seen were shown to be secondary to a primary effect of dietary fluoride on the amount and pattern of food intake.