ANALYSIS OF THE PROLIFERATION KINETICS OF BURKITT'S LYMPHOMA CELLS

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Abstract
The in vitro proliferation kinetics of a cell line derived from a patient with American Burkitt''s lymphoma were investigated at 3 different growth phases: lag (day 1), exponential (day 3) and plateau (day 5). The growth curve, labeling and mitotic indices, percentage labeled mitosis (PLM) curves and DNA content distributions were determined. The data were analyzed by the discrete-time kinetic (DTK) model by which a time course of DNA distributions during a 10 day growth period was characterized in terms of other cell kinetic parameters. The mean cell cycle times, initially estimated from PLM curves on days 1, 3 and 5, were further analyzed by the DTK model and subsequently the mean cell cycle times with respect to DNA distributions during the entire growth period were determined. The doubling times were 39.6, 31.2 and 67.2 h, respectively, at days 1, 3 and 5. The mean cell cycle time increased from 23.0 to 37.7 h from day 3 to day 5 mainly due to an elongation of the G1 and G2 phases. A slight increase in the cell loss rate from 0.0077 to 0.0081 fraction/h was accompanied by a decrease in the cell production rate from 0.0299 to 0.0184 fraction/h. This calculated cell loss rate correlated significantly with the number of dead cells determined by trypan blue exclusion. Analysis of the number of dead cells in relation to the cell cycle stage revealed that a majority of cell death occurred in G1 (r = 0.908; P < 0.0001). There was a good correlation between the in vitro proliferation kinetics at plateau phase of this Burkitt''s lymphoma-derived cell line and in vivo proliferation kinetics of African Burkitt''s lymphoma, suggesting the potential utility of information obtained by in vitro kinetic studies.