Immunochemical Characterization of the Hyaluronic Acid‐Hyaluronectin Interaction

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Abstract
The interaction between calf testis hyaluronic acid (HA) and calf intestine hyaluronectin (HN) was analyzed by gel chromatography and by the effects of HA on the immunological precipitation of HN. This interaction led to formation of larger molecules, as shown by gel permeation. No inhibition of immune precipitation occurred in liquid phase after addition of HA, but the precipitates in unstained gels were rendered transparent, giving the appearance of inhibition. After staining of the gels the precipitates appeared normal. A nonlinear decrease of the diffusion rate in antibody-containing gel was observed as a function of HA concentration at HA:HN weight ratios of 0.75 .times. 10-3 and higher. A faster movement during electrophoresis, depending on the HA:HN ratio, suppressed the precipitation line when tested by electrosyneresis and produced an increase in migration distance when tested by Laurell''s electroimmunoassay. The immunochemical detection and quantitation of HN by these techniques should always consider the amount of HA in the samples. [HN was isolated from human brain.].