The molecular mechanism of interaction of Et3Pb+ with tubulin
- 20 August 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 174 (1) , 128-131
- https://doi.org/10.1016/0014-5793(84)81090-x
Abstract
Triethyllead ion (Et3Pb+) was found to interact with 2 out of 18 thiol groups present in tubulin dimers. Specificity of the interaction was shown by the high affinity of Et3Pb+ to tubulin, by the fact that the 16 residual thiol groups in tubulin remained unaffected, and by the observation that other proteins with exposed thiol groups, e.g., actin, did not react with Et3Pb+. After complexation of the two thiol groups, tubulin in vitro had lost its capability for microtubule assembly. Likewise, polymerized tubulin disassembled on addition of the lead compound.Keywords
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