Cardiac L‐type Ca2+ channel triggers transmitter release in PC 12 cells
- 4 April 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 342 (2) , 209-213
- https://doi.org/10.1016/0014-5793(94)80502-4
Abstract
Among the various voltage-sensitive Ca2+ channels present in PC 12 cells are the dihydropyridine (DHP)-sensitive L-channel, the ω-conotoxin (ω-CgTx)-sensitive N-channel, and an atypical ω-CgTx/DHP-insensitive Ca2+ channel. Depolarization-evoked Ca2+ entry and [3H]dopamine release is mediated by L-type Ca2+ channels determined by the use of Ca2+ channel antagonists, and a single protein of 250 kDa is recognized by L-type-specific antibodies. Screening of a PC12 cDNA library revealed two types of Ca2+channels which were identified by partial sequencing. A pc12-L clone displayed virtually identical sequence homology to the cardiac L-type channel. The identical sequence homology of the single alternative splicing region confirmed clone pc12-L as the rbC-I transcript, a cardiac-neuronal α1 subunit expressed in rat brain. Clone pc12-N displayed identical sequence homology to rbB-I, a neuronal al subunit of the N-type Ca2+ channel expressed in rat brain; Northern blot analysis identified RNA of a size similar to that previously described for rat brain.Keywords
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