CHEMOTAXIS OF RADIOLABELED EQUINE NEUTROPHILS

  • 1 January 1982
    • journal article
    • research article
    • Vol. 43  (3) , 397-401
Abstract
A method for the isolation of equine neutrophils was developed using metrizamide cushions. A purity of > 95% was routinely obtained with > 90% viability. These cells were radiolabeled and tested for their chemotactic response in Boyden chambers to zymosan-activated equine serum, the partially purified equine complement component C5a and formyl-L-methionyl-L-leucyl-L-phenylalanine. The time and ionic requirements for chemotaxis of radiolabeled equine neutrophils were investigated and maximal movement was observed at 2 h incubation and 1.0 mM Ca and 0.5 mM Mg. Dinitrophenol in a concentration range of 10-2-10-6 M also inhibited cell movement. A Zigmond-Hirsch checkerboard assay demonstrated the pronounced chemotactic activity of zymosan-activated equine serum and the partially purified equine C5a. The equine neutrophil did not respond to formyl-L-methionyl-L-leucyl-L-phenylalanine.