Major histocompatibility gene complex (MHC)‐coded determinants on antigen‐specific suppressor factor for delayed‐type hypersensitivity and surface phenotypes of cells producing the factor

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Abstract
Antigen‐specific suppressor factor for delayed‐type hypersensitivity (DTH) to sheep red blood cells (SRBC) was obtained by incubating in vitro spleen cells from CBA mice (H‐2k) injected intravenously 3 days previously with 1 × 109 SRBC. The suppressor factor was characterized for major histocompatibility gene complex (MHC)‐coded antigenic determinants by passing the factor through immunosorbents coupled with appropriate alloantisera. The suppressor factor was absorbed by anti‐H‐2k, anti‐Iakand anti‐I‐Jk immunosorbents but was not retained by anti‐IaS, anti‐I‐JS, anti‐I‐Ak, anti‐I‐E/C′ or anti‐H‐2Kk immunosorbents. In addition, the factor bound to an immunosorbent coupled with rabbit antibodies against carbohydrate‐defined Ia antigens. Furthermore, the suppressive activity that was absorbed was quantitatively recovered in the acid eluates from the immunosorbents. Treatment of the spleen cells with anti‐Lyt‐1.1 antiserum and complement completely abrogated their ability to elaborate the suppressor factor in vitro. In contrast, treatment with anti‐Lyt‐2.1 or anti‐Iak antiserum and complement had no effect. Thus, it appears that the suppressor factor for DTH to SRBC bears I‐J subregion‐coded determinants, and its production is dependent on cells which have the Lyt‐1 +,2− and Ia− phenotype.

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