THE DISTANCE BETWEEN THE PHYTOCHROME CHROMOPHORE AND THE N‐TERMINAL CHAIN DECREASES DURING PHOTOTRANSFORMATION. A NOVEL FLUORESCENCE ENERGY TRANSFER METHOD USING LABELED ANTIBODY FRAGMENTS
- 1 November 1992
- journal article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 56 (5) , 725-733
- https://doi.org/10.1111/j.1751-1097.1992.tb02227.x
Abstract
A novel antibody-fluorescence method has been developed to elucidate the chromophore topography in phytochrome as it undergoes a photochromic transformation. Förster energy transfer from N-terminal bound, fluorescently labeled Oat-25 Fab antibody fragments to the phytochrome chromophore was measured. The results suggest that the chromophore moves relative to the N-terminus upon the Pr-->Pfr phototransformation. This conclusion is consistent with previous models which have proposed a reorientation and an interaction of the Pfr chromophore with the N-terminus. The method described appears to be the first study of a Förster energy transfer measurement using a donor-label attached to a Fab fragment of a photosensor protein.Keywords
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