Enzyme-Linked Immunosorbent Assay for Detection of T-2 Toxin

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Abstract
A microtest plate enzyme immunoassay was developed for the detection of the food toxin, T-2 toxin. Haptenic T-2 hemisuccinate was immobilized on polystyrene surfaces coated with poly(D-Lys). Rabbit antisera against T-2 toxin were added. The binding of the 1st antibody was detected by addition of an anti-rabbit-Ig/peroxidase conjugate. After conversion of the substrate 2,2''-azinobis(3-ethyl-2,3-dihydrobenzothiazole-6-sulfonate)/H2O2, the absorbance at 414 nm was monitored. Presence of free T-2 toxin during the 1st incubation step led to a concentration-dependent lowering of absorbance. The lower detection limit was at .apprx. 2 pg/assay. The cross reactivity of T-2 toxin antiserum with other trichothecenes, as determined by ELISA [enzyme-linked immunosorbent assay], was weaker than that reported by other authors using a radioimmunoassay technique.