DNA rearrangements generating artificial promoters
- 25 June 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 172 (1) , 64-66
- https://doi.org/10.1016/0014-5793(84)80874-1
Abstract
The promoter-cloning plasmid pBRH4 (a derivative of pBR322 with a partially deleted promoter of the tet gene) is shown to contain a sequence which is located near the EcoRI site and can operate as an effective Pribnow box, but is not the remainder of the deletion-inactivated tet promoter of pBR322. If there is a sequence homologous to the ‘−35’ promoter region at the border of the DNA fragment inserted at the EcoRI site, then a compound promoter arises and activates the tet gene. Point mutations in the nonfunctional −35 region of pBRH4 also activate the cryptic Pribnow box. Several compound promoters were obtained through deleting small portions of DNA around the HindIII site of pBR322; the deletions moved various sequences that could operate as Pribnow boxes towards the −35 region of the tet promoterKeywords
This publication has 9 references indexed in Scilit:
- Construction and characterization of E. coli promoter-probe plasmid vectors III. pBR322 derivatives with deletions in the tetracycline resistance promoter regionGene, 1982
- Transcription initiation sites within an IS2insertion in a Gal-constitutive mutant ofEscherichia coliNucleic Acids Research, 1982
- Evidence for two functional gal promoters in intact Escherichia coli cells.Journal of Biological Chemistry, 1981
- Bacteriophage lambda vehicle for the direct cloning of Escherichia coli promoter DNA sequences: feedback regulation of the rplJL-rpoBC operon.Proceedings of the National Academy of Sciences, 1980
- A rapid alkaline extraction procedure for screening recombinant plasmid DNANucleic Acids Research, 1979
- Construction and characterization of E. coli promoter-probe plasmid vectors I. Cloning of promoter-containing DNA fragmentsGene, 1979
- Plasmid vehicles for direct cloning of Escherichia coli promotersJournal of Bacteriology, 1979
- Control features within the rplJL-rpoBC transcription unit of Escherichia coli.Proceedings of the National Academy of Sciences, 1979