Neutral oligosaccharides of bovine submaxillary mucin

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Abstract
Twenty‐two neutral O‐linked oligosaccharides ranging from monosaccharides to octasaccharids were identified in bovine submaxillary‐gland‐mucin glycoprotein by a combination of liquid secondary‐ion mass spectrometery, methylation analysis and 1H‐NMR. Only five of these have been previously detected in bovine submaxillary‐gland mucin although several have been described from other sources of mucin. The structures include short linear sequences 3‐linked to N‐acetylgalactosaminitol (GalNAcol) and branched structures based on either a GlcNAc(β1–6)[Gal(β1–3)]GalNAcol or GlcNAc(β1–6)[GlcNAc(β1–3)]GalNAcol core region. Oligosaccharides not previously characterised from any source were the disaccharide GalNAcα1–6GalNAcol (GalNAc, N‐acetylgalactosamine and the hexasaccharide GlcNAc(β1–6){GalNAc(α1–3)[Fuc(α1–2)]Gal(β1–4)GlcNAc(β1–3)}GalNAcol (Fuc, l‐fucose). Oligosaccharides of the blood‐group‐A type have not been detected previously in bovine submaxillary‐gland mucin although their occurrence on bovine gastric‐mucosal glycoproteins has been established by classical immunochemical studies.

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