Identification of a novel mammalian member of the NSF/CDC48p/Pas1p/TBP‐1 family through heterologous expression in yeast
Open Access
- 5 September 1994
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 351 (2) , 286-290
- https://doi.org/10.1016/0014-5793(94)00879-5
Abstract
Two suppressors of the growth deficiency of a potassium transport mutant of Saccharomyces cerevisiae were isolated from a mouse cDNA expression library. These suppressors, SKD1 and SKD2 (uppressor of + transport growth efect), were cDNAs encoding members of a family of ATPases involved in membrane fusion (N‐ethylmaleimide‐sensitive fusion protein, NSF), cell division cycle regulation (CDC48p), peroxisome assembly (Pas1p), and transcriptional regulation (TBP‐1). The SKD1 protein constitutes a novel member of this family with 49–58% amino acid sequence similarity with other family members, and contains a single ATP binding site. The SKD2 polypeptide is the mouse homolog of NSF.Keywords
This publication has 36 references indexed in Scilit:
- Secrets of Secretion RevealedScience, 1993
- Depletion of intracellular potassium disrupts coated pits and reversibly inhibits cell polarization during fibroblast spreading.The Journal of cell biology, 1993
- Expression of an inward-rectifying potassium channel by the Arabidopsis KAT1 cDNAScience, 1992
- New human gene encoding a positive modulator of HIV Tat-mediated transactivationNature, 1992
- Alterations in a yeast protein resembling HIV Tat-binding protein relieve requirement for an acidic activation domain in GAL4Nature, 1992
- Cloning and Expression in Yeast of a Plant Potassium Ion Transport SystemScience, 1992
- An energy-dependent efflux system for potassium ions in yeastBiochimica et Biophysica Acta (BBA) - Biomembranes, 1991
- The P-loop — a common motif in ATP- and GTP-binding proteinsTrends in Biochemical Sciences, 1990
- Use of a cDNA clone to identify a supposed precursor protein containing valosinNature, 1987
- Depletion of intracellular potassium arrests coated pit formation and receptor-mediated endocytosis in fibroblastsCell, 1983