Cloning and Expression in Yeast of a Plant Potassium Ion Transport System
- 1 May 1992
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 256 (5057) , 663-665
- https://doi.org/10.1126/science.1585180
Abstract
A membrane polypeptide involved in K+ transport in a higher plant was cloned by complementation of a yeast mutant defective in K+ uptake with a complementary DNA library from Arabidopsis thaliana. A 2.65-kilobase complementary DNA conferred ability to grow on media with K+ concentration in the micromolar range and to absorb K+ (or 86Rb+) at rates similar to those in wild-type yeast. The predicted amino acid sequence (838 amino acids) has three domains: a channel-forming region homologous to animal K+ channels, a cyclic nucleotide-binding site, and an ankyrin-like region.Keywords
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