FUNDAMENTAL-STUDIES ON MACROPHAGE-MIGRATION INHIBITORY FACTOR(S) IN THE SUPERNATANT FROM SPLEEN-CELLS IN MICE INFECTED WITH TOXOPLASMA-GONDII

  • 1 January 1979
    • journal article
    • research article
    • Vol. 244  (2-3) , 374-382
Abstract
When migration inhibitory factor (MIF) assay in vitro was conducted on the lymphokines (LK), the percentage of MIF activity was greatly increased 3-4 wk postinfection. In succeeding weeks there was a noticeable decrease in MIF activity noted by the 8th wk postinfection. MIF activity was examined at 1, 6, 12, 18, 24 and 48 h in non-immune spleen cells and in Toxoplasma-immune spleen cells were 2, 21, 29, 54, 70 and 93%, respectively. MIF activity of hyperimmunized spleen cells produced an activity of approximately 50% at 18 h compared to non-immune spleen cells. Characterization of the MIF was performed using Sephadex G-100 and DEAE Sephadex A 50. Two distinct peaks of MIF were identified and separated by Sephadex G-100 gel filtration; they had MW of 30,000-40,000 and 3000-5000, respectively. When the fast peak by Sephadex G-100 was eluted again in DEAE Sephadex A 50, the peak was separated into 4 units, all units showing MIF activity.