Platelet Monoamine Oxidase and Plasma Amine Oxidase: Effect of Anticoagulant and Centrifugation Technique on Platelet Yield and Enzyme Activity

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Abstract
Platelet recovery and activity of [human] platelet monoamine oxidase (MAO) and plasma amine oxidase (PAO) were determined using different centrifugation procedures (125 g for 15 min vs. 600 g for 2.5 min) and anticoagulants. With either centrifugation procedure, the use of EDTA as anticoagulant resulted in higher platelet yields and MAO activity compared to acid-citrate-dextrose (ACD). PAO activity was lower with EDTA as anticoagualnt than with ACD. There was a trend toward higher platelet yields and higher activity levels of MAO and PAO with the 125 g centrifugation method than with the 600 g technique regardless of the anticoagulant used. As MAO and PAO may be of etiologic significance in several psychiatric disorders, implications for MAO studies in psychiatric research were discussed.