Influence of sampling on the interpretation of haematological measurements of rainbow trout, Salmo gairdneri

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Abstract
Hematological parameters of rainbow trout (S. gairdneri) were examined to determine if the sampling influenced the measurements. The results indicated that hematocrit values of fish anesthetized with tricaine methanesulfonate (TMS or MS 222) sampled after 5-20 min were significantly higher than of fish sampled within 1 min of capture and anesthesia. No differences were observed among Hb content, erythrocyte and leukocyte counts or their differential cell ratios over the 20-min period. Hb content and erythrocyte counts increased significantly with size among the 3 groups examined. Differential blood cell ratios apparently could be used as a reliable hematological index because the ratios appear to be independent of the effects of sampling over a short time period.

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