CONCURRENT MEASUREMENT OF PLASMA-LEVELS OF VITAMIN-D3 AND 5 OF ITS METABOLITES IN NORMAL HUMANS, CHRONIC-RENAL-FAILURE PATIENTS, AND ANEPHRIC SUBJECTS (Retracted Article)

  • 1 January 1981
    • journal article
    • retracted article
    • Vol. 98  (4) , 536-548
Abstract
The use of newly developed and established techniques for the determination of plasma levels of a broad spectrum of vitamin D3 metabolites, including vitamin D3 and 25OHD3-lactone, was reported in normal humans, chronic renal failure patients and anephric subjects. The methodology described consisted of methanol-methylene chloride extraction, Lipidex-5000 chromatography with stepwise gradient elution, normal-phase HPLC [high pressure liquid chromatography] with concave gradient elution and sensitive ligand-binding assays. Apparently, there is an extrarenal source(s) for 24,25(OH)2D3 and 25,26(OH)2D3 and both 25OHD3-lactone and 1,25(OH)2D3 may be produced solely in the kidney of the human. Significant reductions or nondetectable plasma levels of vitamin D3 in the renal disease patients may reflect abnormalities in the hepatobiliary-intestinal and/or cutaneous metabolism of vitamin D.