A Novel Monoclonal Antibody Directed Against the Heat-Inducible 68 kDa Heat Shock Protein

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Abstract
A novel monoclonal rat IgM antibody (8F7) is described which detects the inducible 68 kDa heat shock protein (hsp68) in man, mouse, rat, pig and cattle. Hsp68 expression is analysed in various cell types and cell lines by immunoblot with antibody 8F7 and in parallel by autoradiography after metabolic labeling with [3 5S] methionine. The new antibody cross-reacts with proteins of 180 and 48 kDa, which are not heat shock-inducible and show a pattern of expression different than hsp68.