X Chromosome-Linked Transmission and Expression of Retroviral Genomes Microinjected into Mouse Zygotes
- 19 August 1983
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 221 (4612) , 760-762
- https://doi.org/10.1126/science.6683871
Abstract
A genomic clone consisting of the Moloney leukemia proviral genome with moderately repetitive mouse sequences was microinjected into the pronucleus of a mouse zygote. An animal was derived that carried multiple copies of proviral DNA in a tandem array. No evidence for homologous recombination was obtained. The viral genome was expressed in this animal and was transmitted as a single unit to its offspring. Subsequent breeding studies revealed that the proviral DNA had integrated on an X chromosome.Keywords
This publication has 23 references indexed in Scilit:
- Human β-Globin Gene Sequences Injected into Mouse Eggs, Retained in Adults, and Transmitted to ProgenyScience, 1982
- De novo methylation and expression of retroviral genomes during mouse embryogenesisNature, 1982
- X-chromosome inactivation in mammalsCell, 1982
- Regulation of metallothionein–thymidine kinase fusion plasmids injected into mouse eggsNature, 1982
- Integration and Stable Germ Line Transmission of Genes Injected into Mouse PronucleiScience, 1981
- Introduction of a rabbit β-globin gene into the mouse germ lineNature, 1981
- Microinjection of cloned retroviral genomes into mouse zygotes: integration and expression in the animalNature, 1981
- Chromosomal position and activation of retroviral genomes inserted into the germ line of miceCell, 1981
- Integration of Moloney leukaemia virus into the germ line of mice: correlation between site of integration and virus activationNature, 1980
- Retroviruses and embryogenesis: Microinjection of Moloney leukemia virus into midgestation mouse embryosCell, 1980