Regulation of metallothionein–thymidine kinase fusion plasmids injected into mouse eggs
- 1 March 1982
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 296 (5852) , 39-42
- https://doi.org/10.1038/296039a0
Abstract
A plasmid was constructed with the promoter/regulatory region of the mouse metallothionein-I gene fused to the structural gene of herpesvirus thymidine kinase. When mouse eggs were microinjected with this plasmid and incubated with cadmium (a natural inducer of metallothionein gene transcription) thymidine kinase activity increased ∼ 10-fold compared with control eggs not exposed to cadmium. Analysis of a set of deletion mutants revealed that the minimum sequence required for cadmium regulation lies within 90 nucleotides of the transcription start site.Keywords
This publication has 35 references indexed in Scilit:
- Glucocorticoids regulate expression of dihydrofolate reductase cDNA in mouse mammary tumour virus chimaeric plasmidsNature, 1981
- Hormonal inducibility of rat α2u globulin genes in transfected mouse cellsNature, 1981
- Transcriptional regulation of mouse liver metallothionein-I gene by glucocorticoidsNature, 1981
- Regulation of α genes of herpes simplex virus: Expression of chimeric genes produced by fusion of thymidine kinase with α gene promotersCell, 1981
- Hormone-responsive expression of an endogenous proviral gene of mouse mammary tumor virus after molecular cloning and gene transfer into cultured cells.Proceedings of the National Academy of Sciences, 1981
- The binding of a transcription factor to deletion mutants of a 5S ribosomal RNA geneCell, 1981
- Cloned mouse mammary tumor virus DNA is biologically active in transfected mouse cells and its expression is stimulated by glucocorticoid hormonesCell, 1981
- Spacer DNA sequences upstream of the T-A-T-A-A-A-T-A sequence are essential for promotion of H2A histone gene transcription in vivo.Proceedings of the National Academy of Sciences, 1980
- Regulation of simian virus 40 early transcription in vitro by a purified tumor antigen.Proceedings of the National Academy of Sciences, 1980
- MetallothioneinPublished by Springer Nature ,1979