Localization of the human insulin gene to the distal end of the short arm of chromosome 11.

Abstract
The human insulin gene was mapped by hybridization in situ of 3H-labeled recombinant plasmid pHIG900 to chromosome preparations in the presence of 10% dextran sulfate, pHIG900 contains a 900-base pair insert of human genomic DNA that includes the coding region for the C peptide and intervening sequence 2. A significant percentage of hybridized cells (34%) exhibited Ag grains on the distal end of the short arm (band p15) of chromosome 11. Ag grains on this region represented 26% of all chromosomal label. Chromosomal localization of the human insulin gene to 11p15 is demonstrated.