Kinetic analysis in cell culture of the reversal of antiherpes activity of nucleoside analogs by thymidine

1 November 1983

journal article
Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy

Vol. 24 (5) , 819-822
https://doi.org/10.1128/aac.24.5.819

Abstract

The inhibition of herpes simplex virus type 1 plaque formation by acyclovir, bromovinyldeoxyuridine, 9-(3,4-dihydroxybutyl)guanine, and 9-(4-hydroxybutyl)guanine at different thymidine concentrations was analyzed in Lineweaver-Burk plots. Linear competitive patterns between thymidine and the nucleoside analogs were observed for the inhibition of herpes simplex virus type 1 plaque formation. A new constant, the reversal constant Kr, was introduced to describe the sensitivity in cell culture of an antiviral drug to the reversal of its viral activity by a metabolite (e.g., thymidine).

Keywords

This publication has 8 references indexed in Scilit:

Intracellular Pools of Thymidine Reduce the Antiviral Action of Acyclovir
Intervirology, 1983
Comparative efficacy of antiherpes drugs in different cell lines
Antimicrobial Agents and Chemotherapy, 1982
Enzymatic phosphorylation of acyclic nucleoside analogs and correlations with antiherpetic activities
Biochemical Pharmacology, 1981
Antiviral activity of 5-methylthiomethyl-2′deoxyuridine and other 5-substituted 2′-deoxyuridines
Biochemical Pharmacology, 1979
(E)-5-(2-Bromovinyl)-2'-deoxyuridine: a potent and selective anti-herpes agent.
Proceedings of the National Academy of Sciences, 1979
Thymidine kinase from herpes simplex virus phosphorylates the new antiviral compound, 9-(2-hydroxyethoxymethyl)guanine.
Journal of Biological Chemistry, 1978
Antiviral Action and Cellular Toxicity of Four Thymidine Analogues: 5-Ethyl-, 5-Vinyl-, 5-Propyl-, and 5-Allyl-2′-Deoxyuridine
Antimicrobial Agents and Chemotherapy, 1976
The kinetics of enzyme-catalyzed reactions with two or more substrates or products☆II. Inhibition: Nomenclature and theory
Biochimica et Biophysica Acta, 1963