A Rapid Kinetic Assay for Glucose using Glucose Dehydrogenase
- 1 January 1979
- journal article
- research article
- Published by SAGE Publications in Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
- Vol. 16 (1-6) , 100-105
- https://doi.org/10.1177/000456327901600120
Abstract
An automated kinetic assay for the determination of glucose in blood is described. The method employs the enzyme glucose dehydrogenase in the presence of mutarotase, with nicotinamide adenine dinucleotide as hydrogen acceptor. The analytical parameters of the method are determined and the flexibility of the method in relation to sample volume and sensitivity is discussed. Finally, the method is compared with automated glucose oxidase and hexokinase procedures.This publication has 6 references indexed in Scilit:
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- Kinetic Determination of Glucose with the GEMSAEC (ENI) Centrifugal Analyzer by the Glucose Dehydrogenase Reaction, and Comparison with Two Commonly Used ProceduresClinical Chemistry, 1975
- D-Glucose Dehydrogenase from Bacillus megaterium M 1286: Purification, Properties and StructureHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1975