The effects of medium composition and cultural conditions onin vitroroot initiation and growth of papaya (Carica papayaL.)

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Abstract
Reduced mineral concentrations increased root initiation. Media without ammonium produced short, thick roots with few lateral branches. Increased light irradiance and daylength on the foliage increased root numbers and mean root weight per rooted shoot, but light on the basal region of the shoot inhibited root initiation. The addition of riboflavin to the media increased root initiation and root weight, and reduced callus production. Dark incubation reduced callus growth and enhanced the effect of riboflavin on rooting. Four auxins (IAA, IBA, pCPA and NAA) were added individually over a range of concentrations to the basal medium. IBA at 10 μM was the best treatment in terms of root numbers, weight per rooted shoot and quality of root system. NAA and higher concentrations of IBA produced short, thick roots with no lateral branches. The addition of kinetin to the media afforded some protection to the shoots and roots from auxin damage but reduced and delayed rooting.