Effects of Cytokinin and Several Inorganic Cations on the Polyamine Content of Lettuce Cotyledons

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Abstract
Kinetin (4.7 × 10−5M) and 6-benzyladenine (2.22 × 10−5M) were found to increase ca. 2-fold the putrescine content in cotyledons of lettuce (Lactuca sativa L.) seedlings grown for 3 days under fluorescent light. On the other hand, several inorganic ions (K+, Na+, Ga++, Mg++) at a concentration of 3 × 10−2M reduced the putrescine content. The combination of kinetin with one of several inorganic ions at the same level markedly increased the spermine content, but the putrescine content decreased; calcium and magnesium ions were less effective. The physiological significance of these findings is discussed.