Cystatin C in cerebrospinal fluid and multiple sclerosis

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Abstract
Objective A recent study using surface‐enhanced laser desorption/ionization time‐of‐flight analysis of cerebrospinal fluid identified a 12.5kDa truncated isoform of cystatin C (CysC) as a specific biomarker for multiple sclerosis (MS). Methods Surface‐enhanced laser desorption/ionization time‐of‐flight analysis of cerebrospinal fluid samples from 43 MS patients and 46 healthy control subjects. Results Full‐length CysC (13.4kDa) concentration was similar in MS and control samples. The 12.5kDa CysC protein was produced from full‐length CysC by N‐terminal cleavage during storage at −20°C. Interpretation The 12.5kDa CysC isoform is a storage‐related artifact and is not useful as a diagnostic marker for MS. Ann Neurol 2007;62:193–196