Truncated cystatin C in cerebrospiral fluid: Technical artefact or biological process?
- 2 August 2005
- journal article
- research article
- Published by Wiley in Proteomics
- Vol. 5 (12) , 3060-3065
- https://doi.org/10.1002/pmic.200402039
Abstract
Cystatin C, a low molecular weight cysteine proteinase inhibitor present in human body fluids at physiological concentrations, is more expressed in cerebrospinal fluid (CSF) than in plasma. Mass spectrometric characterization showed that after 3 months of storage of human CSF at −;20°C, cystatin C was cleaved in the peptide bond between R8 and L9 and lost its eight N-termini amino acids, whereas this cleavage did not occur when stored at −80°C. This truncation occurred in all CSF samples studied irrespective of the underlying neurological status, indicating a storage-related artefact rather than a physiological or pathological processing of the protein. These results stress the importance of optimal preanalytical storage conditions of any sample prior to proteomics studies.Keywords
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